Abstract
The inhibitory effect of neutral red on plaque formation by foot-and-mouth disease virus, type A, on primary bovine calf-kidney tissue cultures has been studied. The adsorption of virus is complete within 20 minutes at 37°. However, 70 additional minutes of incubation are required to observe maximal titers when 1:10,000 dilution of neutral red is used in the agar nutrient medium. Decreasing the neutral red concentration decreases the incubation time necessary for maximal titers. By appropriately diminishing the concentration of this stain in an agar overlay, the same plating efficiency is obtained as when staining is withheld until after the necrotic areas have developed. It is concluded that some early stages of virus replication are subject to inhibition by neutral red through the action on the host cell, and that after this stage is past, the stain has no detectable effect on further acts of replication.
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