Effect of neuraminidase treatment on the biological activity of highly purified ovine FSH and LH in hypophysectomized immature male and female rats.

Abstract

Highly purified ovine FSH and LH were treated with neuraminidase to remove sialic acid and the desialylated derivatives were examined for biological activity in hypophysectomized immature male and female rats. The male rats were hypophysectomized at 22 days of age and beginning on day 25 were injected sc twice daily for 4 days with native or neuraminidase-treated FSH (total dose, 15 or 60 mug) or LH (12 mug). The ventral prostates, seminal vesicles, and testes were then removed and weighed, and serum testosterone levels were measured by radioimmunoassay. The female rats were hypophysectomized on day 28 and beginning on day 35 were injected sc twice daily for 4 days with native or neuraminidase-treated FSH (8 mug) or saline. On the morning of day 39, the rats were given an ovulating dose of gonadotropin (8 mug native or neuraminidase-treated FSH, or 1.28 mug native or neuraminidase-treated LH) or 1.0 ml saline iv via tail vein. Twenty-four hours later ova were counted in the oviducts the ovaries were weighed, and serum levels of progesterone and 20alpha-dihydroprogesterone were determined by radioimmunoassay. Treatment of ovine LH with neuraminidase did not diminish the ability of this hormone to increase prostate and testes weights and serum testosterone levels. Desialylation also did not decrease the ability of LH to induce ovulation. Although native ovine FSH significantly increased the weights of the ventral prostate, seminal vesicles, and testes, and elevated plasma testosterone levels, the desialylated derivative was essentially inactive. Neuraminidase treatment also eliminated the ability of ovine FSH to increase ovarian weight, to induce ovulation, and to elevate serum progesterone and 20alpha-dihydroprogesterone. These results indicate that the LH-like activity of ovine FSH is an intrinsic property of the FSH molecule.