Effect of Naturally Derived Dentin Bio-modifiers on Immediate and Long-Term Bond Strength of Etch and Rinse Adhesive to Dentin

Abstract

Purpose: To investigate the effect of 10% Proanthocyanidin (PA) rich grape seedextract (GSE) and cardanol oil applied for 60s on immediate and long-term shearbond strength (SBS) of etch and rinse to dentin. Materials and methods: The occlusalsurfaces of forty two samples were ground to expose flat superficial dentin surfaces.The samples were divided into 3 groups; Group A1 (control group) without any pretreatment,Group A2 (10 % GSE), Group A3 (cardanol). Pre-treatments were appliedfor 60s and then washed away for 30s. Each group was further randomly subdividedinto two groups (n=7) according to the storage time (immediate 24 hours) and (longtermsix months period). Shear bond strength (SBS) was tested using a universal testingmachine at a cross head speed of 0.5mm/min. Data was tabulated and presented asmean and standard deviation, one-way ANOVA, post hoc Tukey’s test for pairwise andPaired student t-test was performed between paired groups. Results: All pre-treatmentgroups showed statistically no significant SBS values after 24 hours of storage. Whileat 6 months storage, group A3 showed the highest SBS mean value followed bygroup A2 while Control Group recorded the lowest shear bond strength mean value.Conclusion: Natural dentin Bio-modifiers are considered effective in improving theshear bond strength of the bonded interface and the Bond strength of dentin is affectedby the storage time.