Abstract
The study aimed to investigate the protective action of jatamansi (Nardostachys jatamansi DC.) against doxorubicin cardiotoxicity. Methanolic extract of jatamansi (MEJ) was prepared and standardized using HPTLC fingerprinting, GC-MS chemoprofiling, total phenolic content, and antioxidant activity in vitro. Further in vivo activity was evaluated using rodent model. Animals were divided into five groups (n = 6) namely control (CNT) (Normal saline), toxicant (TOX, without any treatment), MEJ at low dose (JAT1), MEJ at high dose (JAT2), and standard desferrioxamine (STD). All groups except control received doxorubicin 2.5 mg per Kg intra-peritoneally for 3 weeks in twice a week regimen. After 3 weeks, the blood samples and cardiac tissues were collected from all groups for biochemical and histopathological evaluation. Treatment with MEJ at both dose levels exhibited significant reduction (p < 0.001 vs. toxicant) of serum CK-MB (heart creatine kinase), LDH (Lactate dehydrogenase) & HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) levels, and tissue MDA (melondialdehyde) level; insignificant difference was observed (p > 0.05) in TNF-alpha (tumour necrosis factor), IL-6 (interleukine-6) levels and caspase activity as compared to TOX. Histopathological evaluation of cardiac tissues of different treatment groups further reinforced the findings of biochemical estimation. This study concludes that jatamansi can protect cardiac tissues from oxidative stress-induced cell injury and lipid peroxidation as well as against inflammatory and apoptotic effects on cardiac tissues.
Highlights
Doxorubicin (DOX)useful anticancer drugdrug beingbeing used in the management of differentDoxorubicin (DOX)isisa clinically a clinically useful anticancer used in the management of types of cancers including lymphomas and carcinomas [1,2].the major problem problem different types of cancers including lymphomas and carcinomas [1,2]
The 1 mL of DPPH solution (0.004%, w/v) in 95% methanol was taken in test tubes, and 1.0 mL of Methanolic extract of jatamansi (MEJ) was added followed by serial dilutions (5.0–100 μg mL−1 ) to every test tube
The method is based on the principle that sodium nitroprusside in aqueous solution at physiological pH spontaneously generates nitric oxide, which interacts with oxygen to produce nitrite ions; it can be determined by the use of the Griess Illosvoy reaction where scavengers of nitric oxide compete with oxygen, leading to reduced production of nitric oxide
Summary
Doxorubicin (DOX)isisa clinically a clinically useful anticancer used in the management of types of cancers including lymphomas and carcinomas [1,2]. Protecting against doxorubicin-induced cardiotoxicity without affecting the therapeutic value of the jatamansi DC., commonly known as jatamansi, is an important medicinal herb drug Nardostachys of Indian origin, belonging to the family Caprifoliaceae. Have been suggested to protect cells and tissues through their antioxidant properties [20] Various sesquiterpenes such as lignans and neolignans are present in root extracts of plants [19] and Recently, a rodent model of inflammation, jatamansi been shown to properties inhibit the [20]. The protein drug has reported protection doxorubicin-induced inhibiting (Mitogen-activated kinase) activation and against induction of IRF (interferon lipid peroxidation [22,23]; this study is, designed to evaluate thedoxorubicin-induced possible protective role of regulatory factor) [21].
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