Abstract
Summary Callus cultures of wheat ( Triticum aestivum L.) were obtained from segments of root tips and cultivated in the presence of α-naphtylacetic acid (NAA), 2, 4, 5-trichlorophenoxyacetic acid (2,4, 5-T) and 3,6-dichloro-o-anisic acid (Dicamba), respectively. The optimal concentrations for callus growth of the plant growth regulators applied were established. The level of a number of nucleolytic enzyme activities was measured as a function of the intensity of callus growth. With Dicamba, all enzyme activities measured (particularly RNase and DNase) showed a negative correlation with tissue growth. With NAA and 2, 4, 5-T such a correlation was not detected. On the contrary, a positive correlation was observed between tissue growth and two enzyme activities (phosphodiesterase and phosphomonoesterase). The other enzyme activities tended to decrease with higher hormone levels, irrespective of the growth rate.
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