Abstract
The different investigation has been carried out on the biological activities of titanium dioxide nanoparticle but the effect of this nano product on the antibacterial activity of different antibiotics has not been yet demonstrated. In this study the nano size TiO2 is synthesized using citric acid and alpha dextrose and the enhancement effect of TiO2 nanoparticle on the antibacterial activity of different antibiotics was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA). During the present study, different concentrations of nano-scale TiO2 were tested to find out the best concentration that can have the most effective antibacterial property against the MRSA culture. Disk diffusion method was used to determine the antibacterial activity of these antibiotics in the absence and presence of sub inhibitory concentration of TiO2 nano particle. A clinical isolate of MRSA, isolated from Intensive Care Unit (ICU) was used as test strain. In the presence of sub-inhibitory concentration of TiO2 nanoparticle (20 µg/disc) the antibacterial activities of all antibiotics have been increased against test strain with minimum 2 mm to maximum 10mm. The highest increase in inhibitory zone for MRSA was observed against pencillin G and amikacin (each 10 mm). Conversely, in case of nalidixic acid, TiO2 nanoparticle showed a Synergic effect on the antibacterial activity of this antibiotic against test strain. These results signify that the TiO2 nanoparticle potentate the antimicrobial action of beta lactums, cephalosporins, aminoglycosides, glycopeptides, macrolids and lincosamides, tetracycline a possible utilization of nano compound in combination effect against MRSA.
Highlights
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major nosocomial pathogens responsible for a wide spectrum of infections, including skin and soft tissue infections, pneumonia, bacteraemia, surgical site infections (SSI), catheter related infections [1]
The small nanometer-scale TiO2 particles impose several effects that govern its antibacterial action we examined the antimicrobial activity of nanostructured titanium dioxide with different antibiotics against MRSA
The antimicrobial resistance of MRSA against various antibiotics is increased without nano-TiO2 and decreases with nanoscaled TiO2
Summary
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major nosocomial pathogens responsible for a wide spectrum of infections, including skin and soft tissue infections, pneumonia, bacteraemia, surgical site infections (SSI), catheter related infections [1]. Intensive care unit characteristically has higher rates of infections and increased transmission rates, high antibiotic use and large numbers of vulnerable patients [2]. The emergence of bacterial resistance to antibiotics and its dissemination, are major health problems, leading to treatment drawbacks for a large number of drugs [3,4]. There has been increasing interest in the use of inhibitors of antibiotic resistance for combination therapy [5,6]. Nanomaterials are called “a wonder of modern medicine”. It is stated that antibiotics kill perhaps a half dozen different disease-causing organisms but nanomaterials can kill some 650 cells [13]. Resistant strains fail to develop if we apply nanoparticles based formulations in their culture media
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