Effect of N-Acetylcysteine on oxidative stress, nitrosative stress, and viability of broiler blood cells under high ambient temperature

Abstract

Abstract The aim of this experiment was to study the effect of N-Acetylcysteine (NAC) on oxidative and nitrosative stress, and on the viability of broiler blood cells (BBC) when exposed to high ambient temperature. The BBC were maintained at an ambient temperature of 41. 5°C (normal broiler body temperature, NBBT) and compared with high ambient temperatures ranging from 41. 5°C to 46°C (HAT). At HAT, BBC were diluted with NAC at concentrations of 0 (the positive control group, TPCG), 250, 500, 750 and 1000Â μM. Total antioxidant power or ferric reducing antioxidant power (FRAP), malondialdehyde (MDA), hydrogen peroxide (H 2O 2), nitric oxide (NO), and the viability of the BBC were measured. It was found that MDA, H 2O 2, and NO of BBC at NBBT were lower than in the TPCG ( P<Â 0.05). Cell viability of BBC at NBBT was higher than of the TPCG ( P<Â 0.05). At HAT, MDA, H 2O 2, and NO of BBC diluted with NAC at the concentration of 250Â μM were lower than in the TPCG ( P<Â 0.05), and viability of cells of the TPCG was lower than in other groups ( P<Â 0.05). These phenomena showed that NAC could decrease oxidative stress and NO, and could increase the viability of BBC under high ambient temperatures. Finally, a suitable concentration of NAC for BBC was 250Â μM.