Effect of N-acetylcysteine on Nonsteroidal anti-inflammatory drugs induced colitis in rats

Abstract

The aim of this study is to evaluate the effect of NAC on NSAIDs induced colitis in rats. Model of colitis was induced by subcutaneous Indomethacin prepared in 5% sodium bicarbonate administrated at a dose rate of 9 mg/kg for two days at 24 h intervals. NAC (500mg/Kg body weight po) was administrated for seven consecutive days beginning 24 h after the first Indomethacin injection. Colon injury was assessed by body weight loss, colon weight / length ratio, macroscopic damage, histological study, as well as by biochemical measurement of reduced glutathione (GSH), lipid Peroxides and superoxide dismutase (SOD) activity in the colon tissues. The results of this study showed that NAC administration didn't cause decrease in body weight loss, colon weight/length ratio, macroscopic and microscopic colon damage scores caused by administration of Indomethacin. The levels of GSH were decreased. SOD activity was increased and lipid peroxides levels were drcreased in the colon tissue of NAC treated group comparing with Indomethacin control group, but no statistical significance was observed (p>0.05). In this study conclude that NAC didn't ameliorate colitis induced by Indomethacin in this dose regimen.