Abstract
A transepithelial potential of +8.74±0.29 mV ( n = 85) has been recorded across the Malpighian tubules of Locusta. The effect of varying the Na + and K + concentration in the bathing medium on the transepithelial potential has been determined. The data show that the transepithelial potential does not obey the Nernst equation for K +. Ouabain, ethacrynic acid and amiloride all inhibit the transepithelial potential. The results are discussed in relation to the nature of the mechanisms of cation transport across the Malpighian tubules.
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