EFFECT OF MYRTUS COMMUNIS L. ON AN EXPERIMENTAL MODEL OF A RAT LIVER ISCHEMIA-REPERFUSION

Abstract

The Common Myrtle (Myrtus communis L.) is rich in antioxidants, particularly in anthocyanin. It was recognized for its anti-inflammatory, anti-cancer and anti-thrombotic effects. The aim of our work is to evaluate the effect of Myrtle on a model of hepatic ischemia-reperfusion in Rat. Two morphs were chosen: white fruit Myrtle and black fruit Myrtle. Within each morph, fruit and leaf were separated to determine the effect of the extract of each organ. Our work was conducted in three steps: (1) Induction of hepatic ischemia (90 minutes) in Wistar Rat; (2) injection of the extract of the Myrtle 15 minutes before reperfusion; and (3) reperfusion (2 hours). To evaluate the effect of Myrtle on ischemia-reperfusion, we have determined transaminases levels and the concentration of MEGX Monoethylglycinexylidide after a single administration of 1 mg/kg of Lidocaine (for assessing the metabolic capacity of the liver). The determination of total polyphenol extracts of Myrtle showed a significant difference between the black fruit Myrtle 11.32 μg/ml, with white fruit 26.85 μg/ml and the leaves of black fruit Myrtle 94.25 μg/ml. The latter on presented the antioxidant highest (86.54%). With the extract from leaves of Myrtle black fruit, we noted a decrease of AST and ALT, respectively, 1537 U/I and 1008 U/I compared with the negative control which (4969 U/I and 4281 U/I) and an increase in the MEGX 266 μM which was 79 μM at the negative sham. The testing of Myrtle extracts in a model of hepatic ischemia showed a difference in the protective power against damage of ischemia-reperfusion, by origin and type of fruit (black or white).