Abstract
Background and aims: Today, the prevalence of structural and functional disorders of sperm and subsequently the possibility of occurrence of autism, spontaneous abortion, and infertility has increased dramatically. This study aimed to investigate the potential effects of myoinositol and N-acetyl-L-cysteine (NAC) on the improvement of processed spermatozoa indices. Methods: In this study, 63 patients with male factor infertility were studied in Hazrat Zahra Infertility Center of Shahrekord. To do liquefaction, the semen sample was incubated at 37°C for 20 minutes. The Density Gradient Centrifugation protocol was used to process sperm. The processed sperm samples were incubated at 37°C for 20 minutes in four groups of control, myo-inositol (2 mg/mL), NAC antioxidant (10 Mm/L) and myo-inositol+NAC. All biological parameters of sperm were evaluated using Makler slide, SQA-V sperm analyzer gold version 2.48, TUNEL assay, and Toluidine blue staining. Results: In the present study, 63 men aged 22-58 years with infertility complaints were studied. The NAC group and the myo-inositol group exhibited significantly higher motility in comparison with the control group (P<0.001). The NAC group also had a significantly higher number of TUNEL-negative cells and a lower number of TUNEL-positive cells than the control group (P<0.01), but in other variables, the differences were not statistically significant. The morphology, S0, S1, S2 and staining types in the intervention groups did not show any significant difference compared to those in the control group (P > 0.05). Conclusion: NAC and myo-inositol were able to improve certain indices such as sperm motility and integrity; therefore, the quality of sperm could be improved by these drugs to treat infertility.
Highlights
Today, infertility has become one of the main concerns of the community, especially medical circles [1]
Motility rate in the control group significantly changed after sperm processing according to the results of Density Gradient Centrifugation (P < 0.001)
The number of TUNEL-negative cells was significantly higher in the NAC group compared to the control group and the number of TUNEL-positive cells was significantly lower in the NAC group than in the control group, but other groups did not show any significant difference in these two factors
Summary
Infertility has become one of the main concerns of the community, especially medical circles [1]. Regarding the existence of a direct and significant relationship between the deficiency of antioxidants and infertility due to male factors, the use of some antioxidants such as N-acetylL-cysteine (NAC), coQ10, L-carnitine, zinc, selenium, and vitamins C and E, which reduce ROS and increase fertility, is recommended for the treatment of male factor infertility [3]. The processed sperm samples were incubated at 37°C for 20 minutes in four groups of control, myo-inositol (2 mg/mL), NAC antioxidant (10 Mm/L) and myo-inositol+NAC. The NAC group and the myo-inositol group exhibited significantly higher motility in comparison with the control group (P < 0.001). Conclusion: NAC and myo-inositol were able to improve certain indices such as sperm motility and integrity; the quality of sperm could be improved by these drugs to treat infertility.
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