Abstract
ObjectiveTo explore the regulating effect of moxibustion on the enteric nervous system of rats with Crohn's disease. MethodsTen SD rats were selected randomly from 40 rats as normal control (group A), and the other 30 rats were established into Crohn's disease rat models by adopting clysis method with TNBS. On the basis of modeling successfully, the model rats were randomly divided into model group (group B), herbs-partitioned moxibustion group (group C) and mild moxibustion group (group D) with 8 rats in each group (4 rats were dead during modeling. After modeling, 2 rats were selected from group A, and 2 rats were selected from models for determination, at last, 8 rats were included in each group). In group C and group D, herbs-partitioned moxibustion or mild moxibustion was applied on “Tiānshū (▪ ST 25)” bilaterally, and the rats in group A and group B were fixed as in treatment groups. HE stain was conducted, and morphological observation was performed on the colonic tissue of rats; the expression levels of proteins of S-100, SP, NPY and their receptors were observed by adopting immunohistochemical method. ResultsCompared with group A, the expression levels of proteins of S-100, SP and its receptor NK1R, NPY and its receptors NPY1R and NPY2R in the intestinal tract of rats in model groups increased obviously, and the differences were statistically significant. (Ps-100<0.01, PSP<0.05, PNK1R<0.01, PNPY<0.05, PNPY1Y<0.05, PNPY2R<0.01), after treatment with drug-paste interposed moxibustion and mild moxibustion, the levels reduced significantly (PS-100<0.05, PSP<0.05, PNK1R<0.01, PNPY<0.05, PNPY1R<0.05, PNPY2R<0.01). ConclusionMoxibustion treatment may regulate the expression levels of proteins of S-100, SP, NK1R, NPY, NPYIR and NPY2R through warm stimulation, alleviate inflammatory response of colonic tissue, and repair impaired colonic mucosa, thus achieving the goal of treating Crohn's disease.
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