Abstract

The morphogenetic effect of morphactine IT 3456 on meristematic tissue of <i>Cymbidium </i>Sw. was investigated within the dose range 0.01 to 10 ppm in <i>in vitro</i> culture on liquid and agar-solidified medium. In the doses applied, morphactine increased the number of protocorms differentiating from isolated meristematic tissues, and, in dependence on the dose, affected their shape and proliferation. Morphactine retards, and in large doses completely inhibits, the development of rhizoids, shoots and roots. Various developmental anomalies were observed under the influence of morphactine: leaf syncotylia, and their deformation, thickening, shortening, flattening and branching of shoots as well as formation of 2-3 shoots by one protocorm and development of secondary protocorms on the leaves.

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