Abstract
Therapy by Monoclonal antibodies is considered extremely hoping method for cancer therapy. But cancer cells have variable methods for resistance by multiple genetic mutations. The aim of that article to illustrate tagging monoclonal antibodies by gallium containing solamargine glycoside within the antibody by glycosylation the asparagine of its Fc portion. Malignant cells need to a big extent high carbohydrate content for aerobic glycolysis for cancer progression. Solamargine as a specific glycoside can be diffused easily and effectively into malignant cells with a high degree of specificity. Complexion gallium to solamargine then conjugation into monoclonal antibodies will increase Monoclonal antibody potency and affinity by Warburg effect based mechanism and gallium particles. Gallium can be retained for a long time inside malignant cells. By that method, the monoclonal antibody will be targeted to cancer cells by solamargine, retained gallium particles besides its functioning specific Fab region.
Highlights
Introduction in patients withHER-2/neu-positive breast tumor, metastatic gastrointestinal (GI) malignant neoplasms [Pento, 2017]
Its octanol component will be reacted with D-glucopyranose of solamargine rhamnose moiety within the purified glycosylated antibody can be compared with the original form of the same monoclonal antibody type, for example comparing cetuximab solamargine –gallium MAB with the efficacy of Cetuximab on epidermal growth factor expressing cancer cell lines such as colorectal cancer cell lines Cac[0-2], DLD-1, HCT116 and HT-29
Modification of monoclonal antibody with gallium containing solamargine can be a general modification to different types of monoclonal antibodies because it is conjugated on Asn 297 which is a fixed structure to all monoclonal antibodies
Summary
During the haplotype analysis, that included metabolites of plants. They consist of a mono or seven SNPs (rs733618, rs4553808, rs11571317, oligosaccharide chain attached at the C3 position of rs5742909, rs231775, rs3087243 and rs7565213) within the nitrogenous steroid alkaloid backbone [Bill, CTLA4 gene, it can be associated with no response to. The first is the Fab region of monoclonal antibody itself that antagonizes tumor signaling pathways, the second is solamargine and the third is gallium atoms (solamargine salicylate) that will be bound via its octanol domain to solamargine rhamnose moiety using almond β-glucosidase facing cancer cells. At different reaction using suspension adopted CHO-S cells culture and (by times, samples will be withdrawn from the reaction the regular conditions and steps for purification) mixture, put in the freezer for 30 min (monocloanal [Ahmadi et al 2017] and solamargine-glucose (formed in antibody can be stored in -20 ͦ without affecting its step 2), glycosylated monoclonal antibodies will be function [Johnson, 2012], diluted with mobile phase, produced (rhamnose moiety is still not bound) and analyzed by HPLC and spectrophotometer. Reaction (3): Purification of glycosylated monoclonal (glucoside: solamargine glucosyl monoclonal antibody)
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