Effect of Moldey Ion Rhodamine B on viability of Adipose and Bone marrow Derived Mesenchymal Stem cells - an In-vitro Study

Abstract

The study was performed to access the effect of Moldey ion Rhodamine B on in-vitro labelled Bone marrow and adipose derived derived stem cells in sheep. Perirenal adipose tissue and bone marrow from femur were collected from 28 male sheep. Adipose derived mesenchymal stem cells were isolated from perirenal fat using enzymatic digestion method by using type II collagenase. Bone marrow derived mesenchymal stem cells were separated from femur by density gradient centrifugation method using percoll. These mesenchymal stem cells from bone marrow and adipose tissue were cultured and expanded using DulbeccoÂ’s Modified EagleÂ’s Medium with 10 per-cent of fetal bovine serum, one per-cent of antibiotic-antimycotic solution. Adipose and bone marrow derived mesenchymal stem cells from passage 4 to passage 6 were used for labelling with medium containing 25 µg of MIRB per ml. Cell viability was evaluated by Trypan blue exclusion test in both labelled and control (unlabelled) groups. There was no significant difference in the viability of bone marrow and adipose derived mesenchymal stem cells between the labelled and control groups. It was observed that in-vitro labelling of mesenchymal stem cells with MIRB at a concentration of 25 µg per ml of medium did not affect cell proliferation and viability.