Abstract
The effects of modification in sugar concentrations on turgor pressure and membrane potential in epidermal leaf cells of transgenic potato (Solanum tuberosum cv. Desiree) plants were studied. Measurements of turgor pressure were performed by insertion of a micro pressure probe. Osmolality and sugar concentrations were determined by micro analysis of single cell extracts. Membrane potentials and cell diameters were calculated from repeated, computer-controlled scans with voltage-sensitive microelectrodes. Epidermal cells of sucrose transporter antisense plants showed a more than 100% elevation in osmolality and turgor pressure compared to the wild-type. As a consequence, cell diameters were enhanced in this transgenic line. However, membrane potentials were only slightly reduced in sucrose transporter antisense plants. In addition to sucrose transporter antisense lines, transgenic plants that were reduced in their capacity to accumulate starch due to antisense inhibition of the chloroplastic fructose-1,6-bisphosphatase (FBPase) were investigated. These ahtisense plants maintained membrane potential and turgor pressure comparable to the wild-type.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
