Abstract
Optimization of the mobile phase focusing on loading mass for the separation of sanguinarine and chelerythrine by a preparative high performance liquid chromatograph (prep‐HPLC) was investigated. The effects of the mobile phase with modifiers (ion pair, disodium hydrogen phosphate, and triethylamine) on loading mass were compared. Finally, disodium hydrogen phosphate buffer was selected to optimize. The optimum HPLC mobile phase was 0.05 mol/L disodium hydrogen phosphate buffer and acetonitrile (MeCN) (70/30, v/v) (pH = 3–3.5, adjusted by H3PO4). A larger loading mass and volume were obtained in the shorter time. Furthermore, it had been found that the general ion pair RP‐HPLC for alkaloids analysis was not suitable for preparative separation for the components because of its low sample size.
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