Abstract
In this study, mixed electron donors of Mn(II) and Fe(II) were tested for their effects on autotrophic denitrification. The denitrification performance based on various Mn(II)/Fe(II) molar ratios was assessed via batch experiments conducted under different conditions of temperature, pH, and electron donor/electron acceptor molar ratios. Complete nitrate removal was found at high Mn(II)/Fe(II) molar ratios, 30 °C, pH 7.0, and an electron donor/electron acceptor molar ratio of 3.00. Furthermore, we assessed the expression of nirS through amplification approaches and determined N2 levels by gas chromatography. Our results demonstrated that mixed electron donors are efficient for autotrophic denitrification, and strain SZF15 is a suitable candidate for the simultaneous removal of nitrogen, Fe(II), and Mn(II) in groundwater treatment. The present work will benefit the development of autotrophic denitrification technology.
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