Abstract
Tailings used as backfilling material in the presence of mixed bacteria are discussed, and the relationship between mixed bacteria and compressive strength, size variation, water-holding capacity is analyzed in this study. The results illustrate a strong improving response of mixed bacteria with enhanced compressive strength, small size variation and low water-holding capacity of cemented tailings backfill (CTB) specimens. The binder dosage and mixed bacteria proportion have great influence on CTB specimens, which indicate that with the increase of mixed bacteria proportion and binder dosage, compressive strength increased obviously. The maximum compressive strength (4.01 MPa) is obtained in the presence of 100.00% mixed bacteria in contrast to only 2.79 MPa in its absence. Samples added high mixed bacteria proportion yield low water-holding capacity and small size variation. 16S rDNA analysis illustrates that bacteria community is influenced significantly during experiment. Further, possible reaction mechanism is proposed suggesting the possible role of mixed bacteria as promoter to form precipitation (KFe3(SO4)2(OH)6, (NH4)Fe3(SO4)2(OH)6 and (KH3O)4Fe3(SO4)2(OH)6), which reduces tiny cracks in CTB specimens. The technique of using mixed bacteria to reduce binder consumption in this study shows economic benefits to some extent.
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