Abstract
Peripheral blood lymphocytes (PBL) from normal cattle and cattle infected with bovine leukosis virus (BLV) were treated with three different mitogens (phytohemagglutinin, concanavalin A and pokeweed mitogens). Lymphocytes from four cattle infected with BLV had increased spontaneous DNA synthesis. Suppressed mitogen responses were observed in lymphocytes from one leukemic steer with enlarged tumorous lymph nodes. Mitogen responses characterized by low stimulation indexes were also observed in lymphocytes from three other infected animals. The spontaneous DNA synthesis in lymphocytes from BLV infected animals was markedly reduced with BLV antiserum in the culture fluid. BLV production in lymphocyte culture, as indicated by syncytium assays was also inhibited by the antiserum. These inhibitory effects were abolished by absorbing the antiserum with BLV. The results suggest that extracellular BLV as well as BLV antigen on cell surface may be responsible for the increased spontaneous DNA synthesis and the altered mitogen responses of PBL from BLV infected cattle.
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