Abstract

 
 
 
 Purpose: To investigate the effect of long-chain non-coding RNA LINC00491 (LncRNA LINC00491) on the proliferation, migration and invasion of tongue squamous cell carcinoma (TSCC) cells, and the underlying mechanism.
 Methods: Real-time quantitative polymerase chain reaction (qRT-PCR) was applied to determine the expressions of LINC00491 and micro-RNA-384 (miR-384). Furthermore, LINC00491 and miR-384 were transfected into CAL-27 cells, while cell cycle was analyzed using flow cytometry. Cell proliferation was determined by methyl thiazolyl diphenyl-tetrazolium (MTT) assay. Cell migration and invasion were evaluated using Transwell experiments. The relationship between LINC00491 and miR-384 was confirmed using double luciferase reporting assay, while protein expression levels of P21, Ki67, E- cadherin, N-cadherin, and vimentin were assayed with Western blotting.
 Results: The expression of LINC00491 increased in TSCC tissues (p < 0.05). The proportion of cells in G1-phase increased, while the proportion of cells in S-phase decreased (p < 0.05). There was decrease in cell survival, cell migration and cell invasion (p < 0.05). The protein expression levels of Ki67, N- cadherin, and vimentin were lowered, while those of P21, E-cadherin protein were increased (p < 0.05). Transfection of LINC00491 and miR- 384 reduced the proportion of cells in G1 phase, but increased the proportion of cells in S-phase (p < 0.05). Moreover, cell survival, migration and invasion were increased. The protein expressions of Ki67, N-cadherin, and vimentin rose, while those of P21 and E-cadherin decreased (p < 0.05).
 Conclusion: LINC00491 promotes the proliferation, migration and invasion of TSCC cells by inhibiting miR-384. This finding provides a potential target for the treatment of TSCC.
 
 
 
Highlights
Tongue squamous cell carcinoma (TSCC) is one of the common malignant tumors with high degree of metastasis
These results suggest that inhibition of LINC00491 expression may block the proliferation, migration and invasion of TSCC cells, and induce cell cycle arrest in G1 phase
The results showed that the expressions of P21 and Ecadherin were up-regulated in TSCC cells after inhibition of LINC00491 expression, while the expressions of Ki67, N-cadherin and vimentin were down-regulated
Summary
Tongue squamous cell carcinoma (TSCC) is one of the common malignant tumors with high degree of metastasis. Studies have shown that the expression of miR-384, which is low in pancreatic cancer, is involved in tumor cell proliferation [6]. It is not known whether LINC00491 participates in tumorigenesis of TSCC by regulating the expression of miR-384. The purpose of this study was to determine the effect of LINC00491 on the proliferation, migration and invasion of TSCC cells, and its regulatory effect on miR-384. Total RNA was extracted from the frozen TSCC tissues, para-cancerous tissues and transfected CAL-27 cells using TRIzol method.
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