Abstract
Binding of 3H-aldosterone to plasma membranes of adrenalectomized rat kidney has been demonstrated in vivo and in vitro . Steroids with mineralocorticoid activity, deoxycorticosterone or 9-α-fluorohydrocortisone (agonists), or aldosterone antagonists (spironolactone, progesterone) markedly reduced the binding of 3H-aldosterone to renal plasma membranes. Steroids without agonist or antagonist properties (testosterone, 17-β-estradiol) were not effective. The 3H-aldosterone-membrane complex was resistent to solubilization and subsequent chromatography employing Sephadex G-25. These experiments indicate that binding of 3H-aldosterone to adrenalectomized rat kidney plasma membranes may be involved in the cellular mode of action of aldosterone.
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