Abstract
目的 观察亚低温对局灶性脑缺血大鼠缺血局部细胞间黏附分子-1(ICAM-1)表达和血清白细胞介素-6(IL-6)含量的影响,探讨亚低温对脑缺血性损害的神经保护作用机制.方法 将30只体质量在250～300 g的雌性Sprague-Dawley大鼠随机分为亚低温组(n=15)和对照组(n=15),采用线栓法阻断大鼠一侧大脑中动脉制作局灶性脑缺血模型,制模成功后,将亚低温组和对照组大鼠分别置于冰毯机和常温操作台上,使其肛温保持在(33±1)℃和(37±0.5)℃.12 h后,自左心室取血,断头取脑,采用免疫组化方法检测缺血区ICAM-1阳性血管数目和应用免疫放射测定法(IRMA)检测血清IL-6含量.结果 免疫组化分析示缺血局部ICAM-1的表达较对照组下降,IRMA检测的血清IL-6含量亚低温组较对照组降低.结论 亚低温可降低局灶性脑缺血大鼠血清IL-6含量和减少缺血局部ICAM-1的表达,推测亚低温降低IL-6和ICAM-1的表达为亚低温减轻脑缺血性损害的神经保护作用之一。
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