Effect of microwave heating on the phenolic and carotenoid composition and antioxidant properties of Momordicacharantia

Abstract

Momordica charantia L. (MC) is a widely consumed vegetable known for its nutritional benefits, as it is a rich source of carotenoids and phenolic compounds. Various cooking methods are use in domestic settings, including microwave cooking. Therefore, it is crucial to investigate the impact of microwave cooking on the bioactive composition of MC. MC fruits were subjected to microwave heat for 5-, 10-, and 15-min. High performance liquid chromatography was used to identify of carotenoids and phenolic compounds, and total bioactive composition and antioxidant assays were conducted using spectroscopic techniques. There were 17 carotenoids and chlorophylls identified in MC fruit, including lutein, violaxanthin, antheraxanthin, pheophytin a, and all-E-β-carotene. The levels of these compounds significantly increased upon exposure to microwave heating. Similarly, 16 phenolic compounds were identified, and their amounts increased during the treatments, except for 3-hydroxyphloretin-6′-hexoside, quercetin-3-(6″-acetyl)-glucoside, petunidin-3-(6″-acetyl)-glucoside and petunidin-3-(6″-acetyl)-glucoside. The sample subjected to microwave treatment for 15 min exhibited the highest concentration of total phenolic compounds (TPC) at 754.5 mg/100g. The total flavonoid content (TFC) reached 94.6 mg/100g after 10 min of treatment. Additionally, the maximum total anthocyanin content, reported as 54.8 mg/L, was observed in the sample exposed to microwave heating for 15 min.