Effect of microRNA-874 on proliferation and apoptosis in U87 glioma cells

Abstract

Objective To observe the effects of microRNA(miR)- 874 on proliferation and apoptosis in U87 glioma cells. Methods The expression of miR- 874 was detected in 12 cases of gliomas and 6 nontumor control brain tissues by using real- time fluorescent quantitative polymerase chain reaction(FQPCR). The expression of miRNA- 874 was tested by FQ- PCR after synthesized miR- 874 mimics was transfected into U87 glioma cells and cell proliferation was detect by methyl thiazol tetrazolium(MTT).Flow cytometry was applied to detect the cell cycle and apoptosis. Results MiR- 874 was down- regulated in glioblastoma samples compared with non- tumor control brain tissues. The expression of miR- 874 increased significantly after transfection of miR- 874 mimics in U87 cells compared to control group(638.00±80.63 vs. 0.17±0.04,P< 0.05), meanwhile suppressed cells proliferation(48 h:0.357±0.026 vs.0.589±0.021, P<0.05;72 h:0.522±0.034 vs.0.834±0.035, P< 0.05).Up- regulation of miR- 874 raised the percentage of G0/G1 phase cells[(56.28±1.62)% vs.(48.57±0.88)%, P<0.05]and induced cells apoptosis [(12.50±0.66)% vs.(4.91±0.15)%, P< 0.05]. Conclusion The expression of miR- 874 was low in glioblastoma. Loss of miR- 874 is correlated with Tumorigenesis and development. The miR- 874 might be a valid target for the diagnosis and treatment of glioblastoma. Key words: MicroRNA-874; Glioblastoma; Proliferation; Apoptosis