Abstract
Objective To explore the effects of microRNA (miRNA, miR)-483-3p targeting G1S specific cyclin E1 (CCNE1) on the proliferation and migration of colon cancer cells and its mechanism. Methods From June 2015 to June 2019, samples of colorectal cancer and adjacent tissues were collected in our hospital and selected as research objects. The expression levels of miR-483-3p in tumor and adjacent tissues were analyzed by fluorescent quantitative polymerase chain reaction (PCR). Lentiviruses were used to establish control microRNA and miR-483-3p overexpression cell lines (miR-control group and miR-483-3p group) in colon cancer cell lines. The proliferation ability of two groups of cells was analyzed by cell counting kit-8 (CCK-8). The migration ability of two groups of cells were analyzed by Tranwell. bioinformatics and double luciferase reporter gene were used to analyze the target gene of miR-483-3p. The difference between the two groups was analyzed by t test. Results Compared with the adjacent tissues of colon cancer (1.12±0.21), the expression level of miR-483-3p (0.37±0.12) in colon cancer tissues was significantly decreased (t=3.001, P 0.05). Conclusion The expression of miR-483-3p in colon cancer ignificantly down-regulated, which leads to the increase of CCNE1 expression and promotes the proliferation and migration of colon cancer. Key words: MicroRNA-483-3p; Colon cancer; Proliferation; Migration; G1S specific cyclin E1
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
