Effect of microneedles on transdermal absorption of glabridin liniment in vitro

Abstract

Objective To study the effect of transdermal absorption of glabridin liniment with the pig skin pretreated by different microneedles in vitro. Methods The experiment was performed by using HPLC and vertical Franz diffusion cell method with abdominal pig skin as transdermal barrier; all the groups of in vitro accumulative permeation dose, transdermal speedand pig skin residue of glabraidin liniment were measured respectively via sampling at 0.5, 1.0, 2.0, 4.0, 8.0 and 12.0 h, which would be analyzed by comparing the hollow microneedle group and the solid microneedle group with the control group. Results The calibration curve of glabraidin was y =21.43 x + 3.557 8, r=0.999 9, with a linear range in 1.0-100.0 μg/ml. The pig skin drug retention of the hollow microneedle group and the solid microneedle group increased by 64.42% and 39.81% as compared with that of the control group at 0.5 h, respectively. The drug cumulative transmission of the hollow microneedle group and the solid microneedle group were 664.62% and 159.46% higher than that of the control group at 2.0 h. The transdermal rates of the hollow microneedle group and the solid microneedle group produced 131.00% and 66.61% higher than that of the control group. All of the linear equation of accumulative permeation dose were cohere with the zero-order model. Conclusion Both microneedles are conducive to the transdermal absorption of glabraidin liniment, reducing the hysteresis time of transdermal absorption and providing the basis for its application in percutaneous administration. Key words: Glabridin; Microneedles; Vertical Franz diffusion cell method; Transdermal absorption