Abstract
A prospective, multicenter, randomized, sibling oocyte study was conducted with 86 couples to evaluate if a microfluidic sperm separation device improved ICSI sperm selection and subsequent cycle outcomes of fertilization, blastocyst utilization, ploidy, and clinical pregnancy rate when applied to a general patient population. Patients with at least 10 metaphase II oocytes were enrolled in the study and sibling oocyte groups were split in half. One half of the oocytes underwent ICSI with the control processed sperm and the other half were injected with sperm sorted by the ZyMōt microfluidic sperm separation device. Fertilization rate was recorded and resulting blastocysts were biopsied and evaluated for ploidy status with NGS. Euploid, non-mosaic embryos were randomly selected for single embryo transfer. A total of 787 oocytes were evaluated in the ZyMōt group and 777 in the control group. No statistical differences were observed between ZyMōt and control processing methods in any of the study outcomes evaluated. It is possible that the selection of normal, progressive sperm for ICSI, and the repair capacity of oocytes are sufficient to promote normal embryonic development in the general infertility population.
Highlights
The use of intracytoplasmic sperm injection (ICSI) has increased steadily since its successful application in 1992
A total of 787 metaphase II (MII) oocytes were allocated to the ZyMot group and 777 were allocated to the control group
The present study is the first multicenter, prospective, randomized controlled sibling oocyte study to evaluate the effect of the ZyMot microfluidic sperm sorting device on fertilization, blastocyst utilization, euploidy, and clinical pregnancy rates when compared to control sperm processing methods
Summary
The use of intracytoplasmic sperm injection (ICSI) has increased steadily since its successful application in 1992. The selection of normal, highly progressive sperm for ICSI may result in improved fertilization and lower miscarriage rates [2]. Improving these parameters can have a significant effect on the outcome of a cycle for patients seeking fertility treatment. The most commonly employed sperm selection methods include density gradient centrifugation (DGC), direct swim-up, and swim-up wash. Each of these methods has their own limitations. There has been renewed interest in the investigation of alternative methods to isolate motile sperm that result in minimal ROS production and DNA damage
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