Effect of microencapsulated Bacillus subtilis strain CBD2-fermented grain on loperamide-induced constipation in mice

Abstract

Constipation is a common cause of discomfort that affects the quality of life. The aim of the present study was to evaluate the ameliorative effects of microencapsulated food products using Bacillus subtilis CBD2 strain-fermented grain in mice with loperamide-induced constipation. Microcapsules prepared by spray-drying with 2 % pectin and 2 % alginic acid (SD-P2A2) showed higher adhesion and dissolution of amylase than those prepared by freeze-drying. Experimental mice were fed a diet containing SD-P2A2 for 7 days prior to loperamide treatment. The ameliorative effects of an experimental diet containing SD-P2A2 on constipation were compared with those observed in mice fed a control diet containing non-fermented grain. Following the induction of constipation in mice, a marked alteration was observed in fecal parameters (frequency of defecation, fecal weights, fecal water content, and fecal pellet numbers in the distal colon), gastrointestinal transit ratios, harmful enzyme activities, and gene expression differences related to intestinal muscle contraction. These symptoms were significantly ameliorated in mice fed the experimental diet with SD-P2A2 versus constipated control mice. Thus, the intake of microencapsulated food products using B. subtilis CBD2 strain-fermented grain exhibited probiotic effects; these products may be used as a functional material for reducing constipation.