Effect of Microencapsulated 2(3)-Tert-Butyl-4- Hydroxyanisole (BHA) at Sub-Lethal Dose on the Growth of Aspergillus flavus and Production of Aflatoxin in Peanut Food Model System

Daiana Garcia,Natalia Soledad Girardi,Miriam Etcheverry,María Alejandra Passone,Andrea Nesci

doi:10.17756/jfcn.2019-068

Abstract

Fil: Garcia, Daiana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Instituto de Investigacion en Micologia y Micotoxicologia. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigacion en Micologia y Micotoxicologia; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Microbiologia e Inmunologia. Catedra de Ecologia Microbiana; Argentina

Highlights

Peanut (Arachis hypogaea) is a very important dried fruit considered as one of the most widely used nuts due to their nutritional properties and taste
We evaluated growth rate, time to growth, biomass and aflatoxin B1 accumulation of an isolate of A. flavus (RCP08108) with two water activities (0.99 and 0.96 aw) and two growth substrates (Peanut meal extract agar and peanut kernels)
In this sense we evaluated: i) growth parameters based on radial growth and biomass dry weight and ii) aflatoxin B1 (AFB1) accumulation in presence of sub-lethal inhibitory concentrations (0.65 mM) of antioxidant formulation, at two different water activities in peanut meal extract agar (PMEA) and peanut grains

Summary

Introduction

Peanut (Arachis hypogaea) is a very important dried fruit considered as one of the most widely used nuts due to their nutritional properties and taste. The phenolic antioxidant 2(3)-tert-butyl-4hydroyanisol (BHA) has shown effect against moulds and insect, vectors of aflatoxigenic fungi, on stored peanuts [610] In these last publications, the doses applied were always below those allowed by the Codex Alimentarius [11] and the US Food and Drug Administration (FDA) (maximum usage level of 200 mg/g of peanut oil). Our interest is focused on the growth and mycotoxin accumulation produced by A. flavus affected by the exposure to a sub-lethal dose of microencapsulated BHA In this sense we evaluated: i) growth parameters based on radial growth and biomass dry weight and ii) AFB1 accumulation in presence of sub-lethal inhibitory concentrations (0.65 mM) of antioxidant formulation, at two different water activities (aw) in peanut meal extract agar (PMEA) and peanut grains

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Results