Effect of microbial network complexity and stability on nitrogen and sulfur pollutant removal during sediment remediation in rivers affected by combined sewer overflows

Abstract

Discovering the complexity and improving the stability of microbial networks in urban rivers affected by combined sewer overflows (CSOs) is essential for restoring the ecological functions of urban rivers, especially to improve their ability to resist CSO impacts. In this study, the effects of sediment remediation on the complexity and stability of microbial networks was investigated. The results revealed that the restored microbial community structure using different approaches in the river sediments differed significantly, and random matrix theory showed that sediment remediation significantly affected microbial networks and topological properties; the average path distance, average clustering coefficient, connectedness, and other network topological properties positively correlated with remediation time and weakened the small-world characteristics of the original microbial networks. Compared with other sediment remediation methods, regulating low dissolved oxygen (DO) shifts the microbial network module hubs from Actinobacteria and Bacteroidetes to Chloroflexi and Proteobacteria. This decreases the positive association of networks by 17%–18%, which intensifies the competitiveness among microorganisms, further weakening the influence and transmission of external pressure across the entire microbial network. Compared with that of the original sediment, the vulnerability of the restored network was reduced by more than 36%, while the compositional stability was improved by more than 12%, with reduced fluctuation in natural connectivity. This microbial network succession substantially increased the number of key enzyme-producing genes involved in nitrogen and sulfur metabolism, enhancing nitrification, denitrification, and assimilatory sulfate reduction, thereby increasing the removal rates of ammonia, nitrate, and acid volatile sulfide by 43.42%, 250.68% and 2.66%, respectively. This study comprehensively analyzed the succession patterns of microbial networks in urban rivers affected by CSOs before and after sediment remediation, which may provide a reference for reducing the impact of CSO pollution on urban rivers in the subsequent stages.