Effect of metoprolol on serum cardiac troponin I, tumor necrosis factor-α and nuclear factor-κB

Abstract

Objective To investigate the effects of metoprolol on serum troponin I in sepsis rats Cardiac troponin I (cTnI), tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) effect. Methods 40 male SD rats, according to randomly divided into metoprolol group (n=10), model group (n=10), sham operation group (n=10) and normal control group (n=10). In addition to the normal control group was not given the establishment of sepsis model, the other three groups were given the ligation of the perforation of the appendix cecal ligation and puncture (CLP) to establish sepsis model. Sham operation group did not do any treatment, model group after the injection of normal saline through the tail vein. In the metoprolol group after the mold is 2 h through the tail vein of the rats with 0.2 mg/(kg·h) 0.05 mg to speed the impact of the amount of metoprolol to CLP 5 h. And after operation respectively 3, 12 and 24 h by enzyme-linked immunosorbent assay (ELISA) determination of serum cTnI and TNF-α. The changes of NF-κB were observed by using immunohistochemistry semi quantitative method. The pathological changes of myocardial ultrastructure were observed in the myocardial tissue of the rats. Results The postoperative 24 h had no statistical significance in sham operation group and normal control group cTnI, TNF-α NF-κB (P=0.058, 0.124, 0.063). The cTnI, TNF-α and NF-κB levels Metoprolol group were (0.43±0.18) ng/ml, (18.29±2.13) pg/ml, (43.28±2.49) μg/ml, and model group were (0.68±0.14) ng/ml, (20.32±2.16) pg/ml, (51.37±2.61) μg/ml, both of two groups were significantly higher than them of sham operation group (0.12±0.04) ng/ml, (5.54±1.17) pg/ml, (12.43±1.53) μg/ml and control group (0.10±0.01) ng/ml, (5.21±1.12) pg/ml, (11.38±1.65) μg/ml, But cTnI, NF-κB and TNF-α level of metoprolol group were lower than the model group (P=0.042, 0.056, 0.003); And the metoprolol group serum cTnI positively with serum TNF-α (r=0.895, P=0.000), serum cTnI and serum NF-κB positive (r=0.921, P=0.000); Pathological observation of normal control group and sham operation group myocardial ultrastructure had no obvious abnormalities, changes of myocardial ultrastructure in model group and metoprolol group obviously abnormal myocardial ultrastructure changes of mild. Conclusion Metoprolol as a beta blocker, can alleviate myocardial injury in sepsis rats, which may be caused by the inhibition of metoprolol in rat serum cTnI, TNF-α and NF-κB level of inflammatory factors, can be used as an important indicator of early myocardial injury in sepsis and detection the diagnosis. Key words: Metoprolol; Septic disease; Cardiac Troponin I; Tumor necrosis factor-α; Nuclear factor-κB