Abstract
As baccatin III is an immediate diterpenoid precursor of taxol, the increase of baccatin III is beneficial to the biosynthesis of taxol. Addition of methyl jasmonic acid (MJ) enhances the activity of 10- deaceyle baccatin (DAB) III acetyl transferase which catalyzes the bioconversion from 10-DAB III to beccatin III. In this paper, the baccatin III content was increased by 174% by the addition of 100 μmol/L MJ in suspension cultures of Taxus cuspidate. Induction by MJ also increased the expression of a 49.0-kDa protein. This paper describes the cell free acetylation of 10-DAB III in crude extracts of enzyme from the suspension cultures of Taxus cuspidate. The reaction product was confirmed by high performance liquid chromatograph (HPLC). About 25.0% of the 10-DAB III was acetylized into baccatin III on the 4th day with 100 μmol/L MJ. The 10-DAB III acetyl transferase activity reached a peak on the 2nd day with 100 μmol/L MJ, with 54.7% of the 10-DAB III transformed into baccatin III. The baccatin III content increased with the increase of 10-DAB III acetyl transferase activity, although the biosynthesis was delayed by more than 24 h. The remarkable induction of MJ on baccatin III biosynthesis shows a promising way to increase the production of taxol.
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