Abstract
Aims: To investigate the effect of methotrexate and linoleic acid on BAX/BCL 2 ratio in human hepatocyte cell.
 Study Design: Original Research Article.
 Place and Duration of Study: The study was carried out in partnership with the Department of Anatomy and Department of Medical Pharmacology of Çukurova University Faculty of Medicine, using the laboratory facilities of the Department of Medical Pharmacology.
 Methodology: Human hepatocyte cell line (CRL-11233) cells obtained from the American Type Culture Collection Organization (ATCC) were used. Expressions of apoptotic pathway markers, apoptosis inducing factor BAX, BCL2 and BAX/BCL2 were evaluated. All analyzes were examined in four groups (Group 1; control, Group 2; linoleic acid given, Group 3; methotrexate given and Group 4; linoleic acid and methotrexate given).
 Results: The mean ± standard error values of the obtained results as nanogram / milliliter (ng / ml) are in Group I, Group II, Group III and Group IV, respectively; BAX values, 0.900 ± 0.1864, 1.002 ± 0.2098, 8.352 ± 1.467 and 4.295 ± 1.522, BCL 2 values, 13.93 ± 1.198, 13.92 ± 1.739, 2.938 ± 1.059 and 9.250 ± 1.492 and BAX/BCL2 values 0.065, 0.072, 2.843 and 0.464.
 Conclusion: While BAX/BCL2 level increased in the group given methotrexate, it decreased in the group given linoleic acid and methotrexate.
Highlights
The liver is the target organ for drug toxicity as it is responsible for the metabolism of many foreign substances due to its location in the gastrointestinal tract
Human hepatocyte cell line (CRL-11233) cells obtained from the American Type Culture Collection Organization (ATCC) were used
The aim of our study is to investigate the effect of methotrexate and linoleic acid on BAX/BCL 2 ratio in human hepatocyte cell
Summary
The liver is the target organ for drug toxicity as it is responsible for the metabolism of many foreign substances due to its location in the gastrointestinal tract. MTX has been the most commonly used immunosuppressive agent after prednisolone in the treatment of various skin diseases by dermatologists for more than fifty years. It is cheap, has a reducing effect on steroid dose, is well known about its toxicity and side effects, and the availability of efficacy data has increased its use in dermatology [7]. 30% of the patients who receive MTX treatment are discontinued due to drug toxicity [8]. The aim of our study is to investigate the effect of methotrexate and linoleic acid on BAX/BCL 2 ratio in human hepatocyte cell
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
