Abstract
In the present work, the phytochemical screening, polyphenolic content, antibacterial activity and antioxidant activity of Securigera securidaca seeds in methanol were carried out. Phytochemical analysis of seeds showed the presence of alkaloids, flavonoids, saponins, terpenoids, steroids and glycosides. Total phenolic content was estimated by Folin Ciocalteau method and the result showed the highest phenolic content of 62.28 mg/g. Methanolic extract was screened for antibacterial activity by disc diffusion method and it found to be potent. The MIC of methanol extract identified by broth dilution method showed a MIC value of 0.25 mg/ml for both E. coli and Kl. Oxytoca, and also 0.5 mg/ml for both S. aureus and S. epidermis. The antioxidant effect of the seeds was tested by DPPH scavenging activity as in vitro assay. The extract had potent inhibitory activity (IC50) value of 0.057 mg/ml. The finding experimental results showed that methanolic extract of Securigera securidaca is important as a source of antibacterial activity and polyphenolic antioxidants.
Highlights
Medicinal plants have been known as green anticorrosion inhibitors [1,2,3,4,5,6], analgesics, antimicrobial, antioxidant, antispasmodics, and diuretics [7] since ancient times
The odd thing is, no antimicrobial activity of methanol extract, there are many important natural compounds in the crude of S. securidaca seeds and there are many amino acids that have been determined in the composition of Securigera securidaca seeds such as Alanine, asparagine, arginine, aspartic acid, glutamic acid, valine and tyrosine [16,17]
This study (Fig. 1) aims to evaluate the effect of methanolic extract of S. securidaca seeds which will be published for the first time as antibacterial activity against Escherichia coli (E. coli), S. aureus (S. aureus), Staphylococcus epidermis (S.epidermis) and Klebsiella oxytoca (Kl.oxytoca), antioxidant effects in order to use it in some infectious diseases
Summary
Medicinal plants have been known as green anticorrosion inhibitors [1,2,3,4,5,6], analgesics, antimicrobial, antioxidant, antispasmodics, and diuretics [7] since ancient times. It is reported that the ethanolic and aqueous extract of S. securidaca contains various classes of secondary metabolites such as steroids, flavonoids, alkaloids, tannins, cardenolides and penta cyclic triterpenoid type saponins [13,14] Since these compounds may have the potential to inhibit Gram positive and negative bacteria and act as potent antioxidants, they have the ideal chemical structure for scavenging free radicals [15]. This study (Fig. 1) aims to evaluate the effect of methanolic extract of S. securidaca seeds which will be published for the first time as antibacterial activity against Escherichia coli (E. coli), S. aureus (S. aureus), Staphylococcus epidermis (S.epidermis) and Klebsiella oxytoca (Kl.oxytoca), antioxidant effects in order to use it in some infectious diseases
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