Effect of Methanolic Extract of African Ebony (Diospyros mespiliformis) Stem Bark on Liver and Kidney Function Biomarkers in Alloxan Induced Diabetic Albino Rats

Abstract

The effect of methanolic extract of African Ebony (Diospyros mespiliformis) on glucose level, liver and kidney function biomarkers in alloxan induced diabetic rat was investigated. The rats were grouped into four of ten (10) rats each. The rats in Group B, C and D were induced with 0.4mL of alloxan. Group C was treated with 0.3 mL of methanol stem back of Diospyros mespiliformis, Group D was treated with 1.3 mL of metformin; Group A non-diabetic and Group B diabetic untreated. There was a significance increase (P<0.05) in serum glucose level (23.95±1.04mmol/L) of diabetic untreated rat when compared with the normal rat (5.59±0.22mmol/L) Group A. There was also a significant difference P<0.05 in glucose level of the Groups treated with extract (15.90±0.29mmol/L) and Group treated with metformin (17.25±0.28mmol/L) when compared with diabetic untreated rat (23.95±1.04mmol/L). The effect of extract on Liver marker enzymes AST shows a significant (P<0.05) decrease in the Group treated with extract (11.00±0.40U/L) and metformin (11.04±1.08U/L) when compared with untreated (12.88±0.53g/L). ALT showed significant (P<0.05) decrease in the Groups treated with extract (11.05±1.07U/L) and metformin (11.02±1.11U/L) when compared with untreated Group (14.58±0.76g/L). There was a significance P<0.05 increase of Total protein in untreated rats (66.00±2.26g/L) when compared with extract (11.25±1.15g/L) and treated with metformin (11.75±1.11g/L). There was a significance P<0.05 decrease in ALB in diabetic untreated (41.25±0.25g/L) when compared with that treated with extract (57.25±4.37g/L) and metformin (60.20±1.88g/L) and untreated rats. The values of renal function, Urea (6.74±1.76) mMol/l for Group A, (6.50±1.56) mMol/l for Group B, (8.87±1.10)mMol/l for Group C and (8.44±1.21) mMol/l for Group D. Creatinine values (0.44±0.01) mMol/l for Group A, (4.02±0.36) mMol/l for Group B, (3.16±0.10) mMol/l for Group C and (0.46±0.10) mMol/l. The stem bark extracts possesses hypoglycemic effect and also has a positive effect on liver and kidney function biomarker.