Abstract
Bacillus sp. R2purified chitinase was subjected to the effect of metal ions, chemical agents, and organic solvent on its activity, the results showed that K+, Mn2+, Na+, Mg2+ and Ca2+ stimulated chitinase activity by 2,4,7,15 and 21% respectively whereas Cu2+, Fe2+, Zn2+, Ag+ and Hg2+ inhibited the enzyme. 10mM β-mercaptoethanol and EDTA decreased the activity by 38.7 and 78.4% respectively whereas 1% SDS inactivated the enzyme completely, also 1mM galactose, glucose and N-acetyl glucosamine inactivated the enzyme by about 14.4, 25.2 and 38.7% respectively ethanol, acetone and isopropanol inhibited about 30% of chitinase activity however full or nearly full inhibition was observed with chloroform diethylether and ethylacetate. Furthermore the chitinase shelf life was 30 day at -20C and survived 4 freezing and thawing with lost of about 30% of its original activity. This is the first contribution about marine chitinase characterization study from novel gram variable Bacillus isolated from the Red sea.
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