Abstract
The effects of divalent metal cations on DNA polymerase activity of E. coli polymerase I, II or III and on their fidelities during DNA synthesis in vitro have been examined. Carcinogenic or mutagenic metal at the concentration which decreases polymerase activity increased misincorporation, whereas noncarcinogenic metal did not change the fidelity even at a concentration inhibiting polymerase. The change in 3′ → 5′ exonuclease activity of polymerase by metal ions was not responsible for the increase in the misincorporation. The influences of metals on Km for the substrate and template-primer were also observed.
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