Abstract
1. 1. The isolated foot of the brackish-water bivalve, Corbicula japonica, was incubated with or without various inhibitors of energy metabolism under the hypoosmotic and isosmotic conditions. The efflux and the tissue content of ninhydrin-positive substances (NFS) were determined. 2. 2. The NPS efflux increased and their content left in the tissue decreased with decrease in osmotic concentration of the incubation medium. 3. 3. The total sum of NPS released and left in the tissue was not constant; it decreased with decrease in osmotic concentration. 4. 4. A part of free amino acids (FAA) seems to be metabolically transformed into other substances during hypoosmotic stress. 5. 5. The inhibitors of energy production increased NPS efflux from the isolated foot during incubation under the hypoosmotic condition, but not under the isosmotic condition. 6. 6. These inhibitors did not change the NPS content left in the tissue during the both hypo- and isosmotic incubations. 7. 7. These results suggest that intracellular FAA level is determined according to external osmotic concentration, irrespective of the intracellular energy level.
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