Abstract
Objective To invetigate the effect of transplanted umbilical cords mesenchymal stem cells (MSCs) on Wnt/β-catenin signaling pathway in radiation-induced injuried lung tissues of mouse. Methods C57 mice were arranged in normal group, radiation group and MSCs group randomly.Radiation group and MSCs group mice were irradiated with a single dose of 13 Gy X-rays at a dose rate of 3.64 Gy/min by a medical linear accelerator.After irradiation, each mouse of MSCs group received 5×105 MSCs by tail vein injection.And normal and radiation group mice received saline by tail vein injection.On postirradiation 7 day, 30 day and 60 day, lung tissues of mouse were carried out HE pathologic histology examination.The expressions of β-catenin, p-GSK-3β, GSK-3β and FRAT1 were examined by Western bloting.WISP-1 mRNA was detected by real time polymerosea chain reaction. Results The levels of β-catenin, p-GSK-3β/GSK-3β, FRAT1 and WISP-1 in radiation group significantly increased compared to those in normal group on postirradiation 7 day, 30 day and 60 day respectively (P<0.01). Compared to radiation group, the level of β-catenin decreased at each time point, especialy signifcantly on postirradiation 30 day and 60 day respectively (P<0.01). The expression of FRAT1 also decreased at each time point, and decreased significantly on 7 and 30 day postirradiation respectively (P<0.01). The levels of p-GSK-3β/GSK-3β and WISP-1 were all dreased significantly compared to radiation group on postirradiation 7 day, 30 day and 60 day respectively (P<0.01). Conclusions MSCs attenuate the activation of Wnt/β-catenin signaling pathway in radiation-induced injuried lung tissues of mouse. Key words: Wnt/β-catenin signaling; Radiation-induced lung injury; Umbilical cords mesenchymal stem cells
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