Effect of Meloxicam on invasion and migration of liver cancer SMMC-7721 cellS and itsmechanism

Abstract

Objective To explore the effect and mechanism of Meloxicam on migration and invasion of SMMC-7721 cells. Methods Trypan blue dye exclusion assay was used to determine the cytotoxic effect of varying dosages of Mel on the HL-7702 normal human hepaticcells. The migration and invasion of SMMC-7721 cells were determined by wound scratch assay and Transwell™ assay respectively. The expression of cyclooxygenase-2 (COX-2), E-cadherin, N-cadherin, matrix metalloproteinase (MMP)-2 in SMMC-7721 cells treated by Mel were detected by Western blotting. Results 0-200 μmol/ml Mel were not significantly toxic to HL-7702 normal human hepaticcells, 400 μmol/ml Mel had significant cytotoxic to HL-7702 normal human hepaticcells (P=0.007). the abilities of invasion and migration of SMMC-7721 cells were inhibited by Mel. The expression of COX-2 and N-cadherin were decreased but E-cadherin increased and MMP-2 had no significant difference after Mel treatment (P=0.128). Conclusion Mel is less toxic to normal cells, also can inhibit the abilities of proliferation, migration and invasion of SMMC-7721 cells that might be related to regulate the expression of COX-2, N-cadherin and E-cadherin. Thus, the transformation process of epithelial-mesenchymal transition (EMT). Key words: Meloxicam; SMMC-7721 cells; Migration; Invasion