Abstract
The usefulness of various media for the detection of pressure-injured bacteria was studied. Microorganisms, such as Escherichia coli, Vibrio parahaemolyticus, and Listeria monocytogenes were pressurized to obtain a survival rate of 10-50%, and then spread on several kinds of selective and non-selective media to enumerate viable counts. Detection rates on Trypticase soy agar (TSA), Nutrient agar (NA), Anaerobic bacterial count medium (ABCM) and Bonito-peptone-glucose (BPG) medium were generally superior to those on Brain heart infusion (BHI) and Plate count agar (PCA) medium. In the case of Moraxella sp. counts on BHI were only 1/500 of those on TSA. Addition of horse blood to BHI and PCA increased the detection rates. Detection rates on selective medium were relatively low and variable depending on the selective reagents included in the medium.
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