Abstract
he effect of different cultural conditions on production of bioemulsifier from Serratia marcescens S10 was determined; different carbon and nitrogen sources were used such as: different oils include: edible (vegetable) oils (olive oil, sesame oil, sun flower oil and corn oil) and heavy oils (oil 150, oil 60, oil 40) as carbon sources and (NH4Cl, casein, (NH4)2SO4, peptone, tryptone, gelatin and yeast extract) as nitrogen sources were added to production media. Bioemulsifier was estimated by measuring the surface tension (S.T), emulsification activity (E.A) and emulsification index (E24%). The best results of bioemulsifier production from Serratia marcescens S10 were obtained at pH8 and incubated at 37ºC for 5days, using sesame oil as carbon source: surface tension (S.T) was reduced from 67 to 41 mN/m and with emulsification index (E24%) of 92% and emulsification activity (E.A) 0.3 and when used ammonium sulfate as nitrogen source: highest results for the isolate S10: S.T was decreased from 67 mN/m to 24 mN/m, E24% = 88%, E.A = 0.28.
Highlights
Bioemulsifiers or biosurfactants are valuable microbial amphiphilic molecules with effective surface active and biological properties applicable in several industries and processes; environmentally it's more compatible than chemically synthesized rfactants [1]
In general it can be divided into three categories: carbohydrate, hydrocarbons and vegetable oils [3]. [4] noted that the amount of heavy crude oil metabolized by some bacterial species increased with increasing concentration of starter oil up to 0.6% (w/v), the degradation rates appeared to be more pronounced between the concentrations of 0.4 and 0.6% (w/v) oil
This study reports the effect of carbon and nitrogen sources on the production of bioemulsifier by Serratia marcescens S10
Summary
Bioemulsifiers or biosurfactants are valuable microbial amphiphilic molecules with effective surface active and biological properties applicable in several industries and processes; environmentally it's more compatible than chemically synthesized rfactants [1]. 5days, using sesame oil as carbon source: surface tension (S.T) was reduced from to 41 mN/m and with emulsification index (E24%) of 92% and emulsification activity (E.A) 0.3 and when used ammonium sulfate as nitrogen source: highest results for the isolate S10: S.T was decreased from 67 mN/m to 24 mN/m, E24% = 88%, E.A = 0.28.
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