Effect of Matrix Metalloproteinase Inhibitor from Mulberry fruit extract on the Microtensile Bond Strength Stability: An in vitro Study

Abstract

Objectives: The present in vitro study aimed to analyze the effect of two Mulberry fruit extracts on microtensile bond strength of an etch & rinse adhesive immediately and after thermocycling.Materials and Methods: Flat dentin surfaces of 30 sound human molars were bonded with an etch and rinse adhesive. Dentin surfaces were left untreated (control group) or were pretreated with either Morus alba water extract, Morus alba alcohol extract, Morus nigra water extract or Morus nigra alcohol extract. Nano-hybrid resin composite were incrementally built-up. The tooth/composite specimen was serially sectioned in order to produce beams of 0.9±0.1 mm in thickness. Each group was subdivided into two subgroups whether the teeth/composite specimens subjected to thermocycling or not. Each beam was individually fractured by a micro tensile testing machine. The data recorded in MPa, were tabulated and statistically analyzed.Results : Specimens treated with Morus alba and Morus nigra water extracts yielded significantly higher mean bond strengths than alcohol extracts in immediate micro-tensile bond values and after thermocycling. In addition ,Morus alba water extract revealed no statistically significant difference immediately and after thermocycling. Conclusion : Dentin pretreatment with Mulbery water extracts has no adverse effect on the immediate microtensile bond strength and it was able to maintain bond stability of adhesive to dentin.