Abstract
Ischemic encephalopathy is a common clinical disease. The main treatment goal is to achieve vascular recanalization. However, after vascular recanalization, the reperfusion of fresh blood can change local cell metabolism, thus adversely affecting cell structure and function, which can result in reperfusion injury. To explore the effect of matrine intervention of different concentrations on JAK2/STAT3 signaling pathway and brain protection in rats with cerebral ischemia-reperfusion. Healthy male Sprague Dawley rats were divided into a blank control group (20 rats), a model group (80 rats) and a sham group (20 rats). In the model group, the middle cerebral artery was occluded with suture method to establish cerebral ischemia-reperfusion model rats, which were subdivided into cerebral ischemia-reperfusion group, and 5, 10 and 20 mg/kg matrine groups, with 20 rats in each group. Indicators including neurological function score, brain infarct size, brain water content, lactic dehydrogenase activity, protein expressions of p-JAK2 and p-STAT3, as well as superoxide dismutase activity and malondialdehyde content were evaluated. Compared with cerebral ischemia-reperfusion group, all the indicators were significantly improved in the 3 matrine treatment groups in a dose-dependent manner, and protein expressions of p-JAK2 and p-STAT3 in the brain tissue and brain cell apoptosis rate were decreased with the increase of matrine concentration (all p < 0.05). Matrine can significantly ameliorate the neurological function and brain edema of rats with cerebral ischemia-reperfusion, and improve superoxide dismutase, malondialdehyde and lactic dehydrogenase levels in the brain tissue and brain cell apoptosis rate. The mechanism of matrine may be related to the inhibition of abnormal JAK2/STAT3 signaling pathway activation.
Highlights
Reperfusion injury is common in ischemic encephalopathy.[1,2] After the occurrence of cerebral ischemia-reperfusion, brain cells will gradually die in the form of apoptosis; the occurrence of cerebral ischemia-reperfusion leads to neuron necrosis and neurological deficit.[3,4]Matrine is a kind of monomeric compound extracted from Sophora flavescens, a legume plant
After vascular recanalization, the reperfusion of fresh blood can change local cell metabolism, adversely affecting cell structure and function, which can result in reperfusion injury
Compared with cerebral ischemia-reperfusion group, all the indicators were significantly improved in the 3 matrine treatment groups in a dose-dependent manner, and protein expressions of p-JAK2 and p-STAT3 in the brain tissue and brain cell apoptosis rate were decreased with the increase of matrine concentration
Summary
Reperfusion injury is common in ischemic encephalopathy.[1,2]. Matrine is a kind of monomeric compound extracted from Sophora flavescens, a legume plant. It has anti-tumor and anti-oxidation effect, and protects the cardiocerebral vascular system from injuries to some extent.[5,6]. As for the effect of matrine on cerebral ischemia-reperfusion, some studies suggested that matrine could exert a protective effect on neurons of rats with cerebral ischemiareperfusion by activating cannabinoid receptor type 2.8. Ischemic encephalopathy is a common clinical disease. After vascular recanalization, the reperfusion of fresh blood can change local cell metabolism, adversely affecting cell structure and function, which can result in reperfusion injury
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
