Effect of Marine Collagen Peptides on Physiological and Neurobehavioral Development of Male Rats with Perinatal Asphyxia.

Linlin Xu,Yajun Xu,Wenhong Dong,Jie Zhao

doi:10.3390/md13063653

Abstract

Asphyxia during delivery produces long-term deficits in brain development. We investigated the neuroprotective effects of marine collagen peptides (MCPs), isolated from Chum Salmon skin by enzymatic hydrolysis, on male rats with perinatal asphyxia (PA). PA was performed by immersing rat fetuses with uterine horns removed from ready-to-deliver rats into a water bath for 15 min. Caesarean-delivered pups were used as controls. PA rats were intragastrically administered with 0.33 g/kg, 1.0 g/kg and 3.0 g/kg body weight MCPs from postnatal day 0 (PND 0) till the age of 90-days. Behavioral tests were carried out at PND21, PND 28 and PND 90. The results indicated that MCPs facilitated early body weight gain of the PA pups, however had little effects on early physiological development. Behavioral tests revealed that MCPs facilitated long-term learning and memory of the pups with PA through reducing oxidative damage and acetylcholinesterase (AChE) activity in the brain, and increasing hippocampus phosphorylated cAMP-response element binding protein (p-CREB) and brain derived neurotrophic factor (BDNF) expression.

Highlights

Perinatal asphyxia (PA) has been associated with a wide spectrum of short-term or long-term neurobehavioral disorders [1,2,3]
WBWG of pups in 1.0 and 3.0 g/kg body weight marine collagen peptides (MCPs) intervention groups were significantly increased compared with the PA control group (p < 0.05 and p < 0.01, respectively)
MCP intervention with 1.0 and 3.0 g/kg showed protective effects, as the WBWG was significantly increased in these two groups compared with the PA control group (p < 0.05)

Summary

Introduction

Perinatal asphyxia (PA) has been associated with a wide spectrum of short-term or long-term neurobehavioral disorders [1,2,3]. Bioactive peptides that are present in the amino acid sequence of food proteins have become of particular interest in nutrition and food science in recent decades. These peptides, which are inactive within the sequence of the parent protein, can be released by enzymatic proteolysis, for example during gastrointestinal digestion or during food processing [9]. It is possible that the small peptides can reach the target organs to exert their beneficial biological effects. The bigger peptides are broken down into amino acids and/or small peptides in the gut to be absorbed and exert their function

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