Effect of malachite green, ocimum sanctum, and Er, Cr: YSGG laser on antimicrobial activity against S.mutans and CAD disinfection bonded to resin restoration

Abstract

AimsEvaluation of survival of S.mutans and shear bond strength (SBS) of adhesive restorative material bonded to carious affected dentin (CAD) after using cavity disinfectant i.e., Er, Cr: YSGG (ECYL), (Nd: YAG) (NYL), Malachite green (MG), Ocimum sanctum (OS) in comparison to the conventional control chlorhexidine gluconate (CHX). Materials and methodsSeventy-five caries-affected mandibular third molars having ICDAS scores 4 and 5 were included. Carious infected dentin was eliminated and CAD surface was obtained. On twenty-five samples, an uncontaminated suspension of S. mutans strain ATCC 35,668 in Brain-Heart Infusion broth (BHI) was obtained after 12 h of incubation at 37 °C. Based on the disinfection regime used to sterilize the CAD surface, all the specimens were allocated into five different groups. (n = 15) Group 1: CAD disinfected with CHX, Group 2: CAD disinfected with ECYL, Group 3: CAD disinfected with NYL, Group 4: CAD disinfected with MG, and Group 5: CAD disinfected with OS. An assessment of the survival rate of S. mutans was performed. Fifty specimens were smeared with single-step adhesive bond followed by restoring the CAD surface using packable composite. SBS and failure mode were determined using a universal testing machine and stereomicroscope at 40x magnification. ANOVA and post-hoc Tukey-Kramer test was used for data analysis p = 0.05. ResultsGroup 1: CHX-treated specimens displayed the lowest bacterial viability (0.11±0.03 CFU/mL). However, group 4: MG exhibited the highest bacterial survival (0.15 ± 0.09 CFU/mL). The highest and lowest SBS of resin adhesive bonded to disinfected CAD surface were exhibited by (group 5: OS) (21.48±1.39 MPa) and (group 3: NYL) (16.41±0.90 MPa) respectively (p>0.05). ConclusionOcimum sanctum seems to be promising in terms of its antibacterial efficacy against S.mutans and positive impact on SBS of resin adhesive to CAD surface.