Abstract
Some enzymes of seminal plasma such as, Glutamic oxaloacetic transaminase, Glutamic Pyruvic transaminase, Acid phosphatase and Alkaline phosphatase are essential for metabolic processes which provide energy for viability, motility and fertility of spermatozoa. These enzymes are used as good indicators of semen quality as they measure the plasma membrane stability of spermatozoa, so the aims of study are to estimate the enzymes level and total protein in seminal plasma of Holstein bulls. In present the available knowledge there is no study in Iraq regarding the effect of using magnetically treated water on enzymes and total protein in seminal plasma of Holstein bulls born in Iraq to improve the Artificial Insemination programs. Sixteen bulls were divided equally into two groups depending on their age. Young (17-19 months) and adult (29-32 months), then randomly distributed into two equal sub groups (control and treatment) through February until June months. Treatment group was given magnetically treated water which passed through magnetic device with strength of 2000 gauss; control group was given normal water without magnetism. Results show that there is a monthly variation in levels of enzymes, in the group drinking magnetic water effect significantly (P<0.05), all enzymes of seminal plasma of Holstein bulls in Iraq, with no effect on total protein.
Highlights
Results show that there is a monthly variation in levels of enzymes, in the group drinking magnetic water effect significantly (P
A comprehensive of water from body because decrease review on biochemical characteristics and superficial tying in water [9], surface tension functions of seminal plasma proteins has [10], changes in pH [11] and other physical recently been published by [3], where the properties such as increase ability of body for Authors have discussed elaborately the production general hormones [12,13,14] and protective capability of some low molecular increase absorption [10] .In another hand this seminal plasma proteins against cold shock of changes in magnetic water gives benefits spermatozoa and antioxidant properties. The animals such as improved semen determination of protein content on sperm characteristics, Fertility rate [15], sexual hormones [14], enzyme (8 and 16), Blood picture [17], There are no study in Iraq regarding the effect of using magnetically treated water on enzymes and total protein in seminal plasma of Holstein bulls born in Iraq to improvement the Artificial Insemination programs in Iraq
After application of few drops of semen needed for variation parameters for evaluation of semen quality, one ml of semen was centrifuged for 30 minute at 4000 rpm at 10°C to separated seminal plasma and checked under microscope to insure that it was sperm free, it was transferred to sterile vials and stored at -20°C for enzymes and protein analysis
Summary
Some enzymes of seminal plasma such as, Glutamic oxaloacetic transaminase, Glutamic Pyruvic transaminase, Acid phosphatase and Alkaline phosphatase are essential for metabolic processes which provide energy for viability, motility and fertility of spermatozoa. These enzymes are used as good indicators of semen quality as they measure the plasma membrane stability of spermatozoa, so the aims of study are to estimate the enzymes level and total protein in seminal plasma of Holstein bulls. In present the available knowledge there is no study in Iraq regarding the effect of using magnetically treated water on enzymes and total protein in seminal plasma of Holstein bulls born in.
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