Effect of magnesium on the osteogenesis of normal human osteoblasts.

Abstract

Biomaterials containing magnesium are used for implants and bone regeneration. However, mechanisms underlying the biologic effects of magnesium are still largely unknown and have not been examined on normal human osteoblasts. This study was designed to test the effect of supplemented Mg2+ concentrations between 0.5mM and 16mM on the osteogenic behaviors of normal human primary osteoblasts. Human primary osteoblasts were cultured in the groups with various concentrations of supplemented magnesium for various time intervals. Cell proliferation was measured using crystal violet staining. Degree of Alkaline Phosphatase (ALP) activity was measured by fluorometric assay. Expression of osteocalcin was measured by immunosorbent assay. Mineralization of cultures was determined by Alizarin Red S staining. Results showed that initial cell attachment efficiency was not affected by supplemented Mg2+ (P>0.05). At 21 days, proliferation rates increased in groups containing 0.5mM-4mM supplemented Mg2+ and decreased in groups of supplemented 8mM and 16mM Mg2+. ALP activity and osteocalcin expression were upregulated in groups of supplemented Mg2+ between 0.5mM-2.0mM (P<0.05), but downregulated in groups with supplemented Mg2+ concentrations of 4mM and above (P<0.05). Cultures with 1mM and 2mM supplemented Mg2+ showed upregulated mineralization activity compared to the control (P<0.05), but downregulated in groups with supplemented Mg2+ concentrations of 4mM and above (P<0.05). The present study based on an experimental design demonstrated the impact of 2mM supplemented Mg2+ on induced-proliferation and differentiation of normal human osteoblasts.