Abstract
Summary The effect of macerozyme on accumulation of indole alkaloid and coumarine and on the induction of tryptophan decarboxylase (TDC, E.C. 4.1.1.28), phenylalanine ammonia-lyase (PAL, E.C. 4.3.1.5) and phosphatydilinositol 4,5-bisphosphate phospholipase C (PLC, E.C. 3.1.4.3) activities on Catharanthus roseus hairy roots was investigated. Increasing concentrations of macerozyme induced an increase in indole alkaloid and coumarine accumulation. There was a 10-fold increase in TDC and a 4-fold increase in PAL activities in hairy roots treated with 1 % of macerozyme. In a dose-response experiment PLC activity decreased 38 % with 0.5 % of macerozyme and increased 40 % with 1 % of macerozyme when compared to untreated roots. The results demonstrate that macerozyme treatment can affect the production of secondary metabolites and PLC activity in C. roseus hairy roots.
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